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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Acta Biomater. 2017 Sep 13;63:50–63. doi: 10.1016/j.actbio.2017.09.011

Figure 6. Phenotypic characterization of cells in ECM hydrogel.

Figure 6

A Immunohistochemical characterization of individual cell phenotypes within the ECM hydrogel at different magnifications. B. A phenotypic analysis of cells present within the hydrogel were predominantly microglia (ionized calcium binding adapter molecule 1, Iba-1), as well as fewer (p<0.01) cells from the oligodendrocyte lineage (2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNPase). Significantly (p<0.001) fewer neurons (Fox3), neural progenitors (doublecortin, DCX), astrocytes (glial fibrillary acid protein, GFAP) or endothelial cells (rat endothelial cell antigen 1) were found. C. A comparison of monocyte polarization indicated that these mostly co-expressed M1 (CD86) and M2 (CD206) markers, with significantly fewer cell expressing only CD86 (p<0.01) or CD206 (p<0.001). Scale bars 100 μm.