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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Neurobiol Aging. 2017 Sep 21;60:129–140. doi: 10.1016/j.neurobiolaging.2017.08.024

Figure 2. The Par3/aPKC complex promotes BACE1 phosphorylation on Ser498.

Figure 2

(a) Hippocampal neurons were transfected with BACE1-FLAG and immunostained for FLAG (red) and TGN (green). Scale bar: 10 μm. (b) Quantification of colocalization of BACE1-FLAG and TGN. Data were expressed as Mean ± SEM with Student’s t test, ***p<0.001, n=15. (c) Primary hippocampal neurons were infected with Luciferase shRNA or Par3 shRNA expressing lentivirus at DIV 0. On DIV 7 neurons were lysed and analyzed by Western blotting. (d) Primary hippocampal neurons were treated with aPKC pseudosubstrate inhibitor (PSI) (2μg/ml) at DIV 10 and lysed on DIV 13. Lysates were analyzed by Western blotting to detect total BACE1 and phospho-Ser498 BACE1 levels. (e) Quantification of pSer498 normalized to total BACE1 from blots in (c). (f) Quantification of pSer498 normalized to total BACE1 from blots in (d). Data were expressed as Mean ± SEM with Student’s t test, *p<0.05; n=6.