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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Neurobiol Aging. 2017 Sep 21;60:129–140. doi: 10.1016/j.neurobiolaging.2017.08.024

Figure 6. Depletion of PACS1 causes a redistribution of BACE1 constructs to the late endosomes/lysosomes.

Figure 6

(a) Hippocampal neurons were transfected with indicated constructs. At DIV10, neurons were immunostained for FLAG (magenta) and TGN (cyan). Merge shows the overlap between BACE1 and TGN. Venus (green) indicates positive neurons. (b) Hippocampal neurons were transfected with indicated BACE1 constructs (magenta) and live stained with Lysotracker Deep Red (cyan) for 5 min. Merge shows the overlap between BACE1 and Lysotracker. Venus (green) indicates positive neurons. (c) Quantification of BACE1-FLAG and TGN colocalization. (d) Quantification of BACE1-RFP and Lysotracker Deep Red colocalization. Data were expressed as Mean ± SEM with Student’s t test: *p<0.05; ** p<0.01; n=10–15. Scale bar: 10μm.