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A screenshot of the sequencing pile-up at EGFR codon 790 from a pretreatment lung adenocarcinoma sample with an EGFR L858R mutation (not shown). One read of c.2369C > T (p.T790M) is visible here (blue arrow) out of a total of 110 reads (<1% mutant allele fraction). This finding could not be reproduced using a highly sensitive ddPCR assay and thus is attributed to artifact.
