Figure 2.

In vitro sensitivity assay for candidate agents. (A) NR4A2 expression at protein level was assessed by SDS-PAGE electrophoresis. CRTC1-MAML2 + (H3118) and LKB1 mutant cells (H2126) showed high NR4A2 expression; (B) mRNA expression of NR4A2 was also tested for the corresponding human cell lines; (C) cells were incubated with candidate agents for 48 hours at indicated concentration in 96 well plates. Differences in sensitivity between H3118 and H2087 cells at 1 µM were assessed using two sided, Wilcoxon rank-sum test. Stars indicate P<0.05.