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. 2017 Oct 24;8:1104. doi: 10.1038/s41467-017-01131-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Irisin induces gene expression changes and improves myogenesis. a Image of Coomassie-stained polyacrylamide gel showing recombinant His-tagged irisin protein. Recombinant irisin protein was detected as a single band at ~15 kDa. Lane 1 shows the SeeBlue Plus 2 Pre-Stained ladder. Lanes 2, 3, and 4 show 1, 2, and 5 μl of the purified His-tagged irisin protein, respectively. b Graph representing qPCR analysis of Ucp1 expression in human subcutaneous white adipose-derived stem cells (hADSCs) after 21 days of adipogenic differentiation in the presence of DB (control) or irisin (n = 2 biological replicates). c Graph representing qPCR analysis of Ucp1 in 3T3L1 fibroblasts after 4 days of adipogenic differentiation in the presence of DB (control) or irisin (n = 3 biological replicates). Graphs displaying qPCR analysis of sox8 (d), heyL (e), haptoglobin (f), il6 (g), cxcl1 (h), and ptx3 (i) in 72 h-differentiated C2C12 myotubes treated with DB (control) or recombinant irisin protein for 0, 6, 12, 24, and 48 h. All qPCR graphs show gene expression normalized to gapdh (n = 3 biological replicates). Error bars represent mean ± SEM. Student’s t-test was performed for b and all relevant figure panels between d and i, and one-way ANOVA was peformed for c. Significance is indicated with *p < 0.05, **p < 0.01 and ***p < 0.001