Fig. 3.

Irisin enhances myogenesis in primary human myoblast cultures. a Representative images of H&E-stained 36C15Q primary myoblasts at 24, 48, 72, and 96 h of differentiation in the presence of DB (control) or irisin (250 and 1000 ng/ml). b Graph showing quantification of myotube number at 0, 24, 48, 72, and 96 h of differentiation in the presence of DB (control) or irisin (250 and 1000 ng/ml). c Graph showing quantification of fusion index at 0, 24, 48, 72, and 96 h of differentiation in the presence of DB (control) or irisin (250 and 1000 ng/ml) (n = 2 biological replicates). Error bars represent mean ± SEM. One-way ANOVA was performed for all relevant figure panels. Significance is indicated with *p < 0.05 and **p < 0.01