Table 2.
Primer | Sequence (5′-3′) | Annealing temp (°C) |
---|---|---|
341F-GC | CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGGCCTAC GGGAGGCAGCAG |
65–55a |
907R | CCGTCAATTCCTTTGAGTTT | 65–55a |
0357F | CCCTACGGGGCGCAGCAG | 69 |
0915aR | GTGCTCCCCCGCCAATTCCT | 69 |
0357F-GC | CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGCCCTAC GGGGCGCAGCAG |
57 |
0691R | GGATTACARGATTTCAC | 57 |
a These primers were used in a touchdown protocol where the annealing temperature decreased from 65 to 55 °C in 20 cycles