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. 2017 Oct 23;7(6):375. doi: 10.1007/s13205-017-1009-x

Table 2.

Primers used in the study

Primer Sequence (5′-3′) Annealing temp (°C)
341F-GC CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGGCCTAC
GGGAGGCAGCAG
65–55a
907R CCGTCAATTCCTTTGAGTTT 65–55a
0357F CCCTACGGGGCGCAGCAG 69
0915aR GTGCTCCCCCGCCAATTCCT 69
0357F-GC CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGCCCTAC
GGGGCGCAGCAG
57
0691R GGATTACARGATTTCAC 57

a These primers were used in a touchdown protocol where the annealing temperature decreased from 65 to 55 °C in 20 cycles