Figure 5.

miR-210 overexpression reduced hypoxic cell injury and apoptosis in HK-2 cells, while miR-210 knockdown exerted the opposite effect. We transfected miR-210 mimic and inhibitor into HK-2 cells and conducted LDH and annexin V/PI assays after normoxia (21% O₂) or hypoxia (0.3% O₂) treatment for 48 h. LDH release was evaluated in cell medium after (A) miR-210 overexpression or (B) miR-210 knockdown in HK-2 cells treated with hypoxia. Cell apoptosis was analyzed in the (C) miR-210 mimic group and (D) miR-210 inhibitor group using annexin V/PI staining flow cytometry assay. Units of the Y and X axes are fluorescence intensity. Annexin V (+) PI (+), annexin V (+) PI (−), annexin V (−) PI (+) and annexin V (−) PI (−) indicated late apoptotic, early apoptotic, necrotic and living cells, respectively. All data are repeated from three independent experiments (n = 3). Data are shown as mean ± SD. NC, negative control. *P < 0.05; **P < 0.01; ***P < 0.001.