Fig. 4.

Amounts of ellipticine metabolites generated by human CYP3A4 in the presence of NADPH or NADH with or without cytochrome b ₅ (at a molar ratio of CYP3A4:cytochrome b ₅ of 1:5). Data shown are mean peak areas relative to the internal standard phenacetin ± SD (n = 3; analyses of three independent in vitro incubations). ND not detected. ^∆∆∆ P < 0.001, ^∆ P < 0.01 (Student’s t test), significantly different from incubations in the presence of NADPH as a cofactor; ***P < 0.001 (Student’s t test), significantly different from incubations without cytochrome b ₅. Control—incubations without added cofactors