Figure 5.

MID1 immunostaining of the temporal cortex from human control and patients with hyperphosphorylated Tau and Aβ plaque deposition. (a–c) MID1 immunohistochemistry. MID1 is shown in brown and the nuclei are denoted with eosin (blue). (a) Very little MID1 signal is observed in the control (no Alzheimer’s pathology or related clinical signs at the age of 79 years). (b) MID1 immunostaining is clearly present in patient 1, who was diagnosed clinically with AD and showed pathology of hyperphosphorylated Tau and intracellular Aβ plaque deposition (age 65 years). (c) Substantial MID1 signal is observed in patient 2, who had no clinical signs of AD at the age of 61 years, but showed significant pathology of Aβ plaques and neurofibrillary tangles. Scale bar = 200 µm. *Denote cells that were enlarged in the inset of each panel. (d–l) MID1 immunofluorescence staining. MID1 is stained in red, nuclei are visualized with DAPI (blue). (d,g,j) Very little MID1 signal is observed in the control. (e,h,k) MID1 immunostaining is clearly present in patient 1. (f,i,l) Substantial MID1 signal is observed in patient 2. Scale bar = 25 µm. (m) Quantification of MID1 signal intensity of samples shown in (d–l).