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. 2017 Oct 23;2:30. doi: 10.1038/s41541-017-0030-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Structure resolution of engineered CMV VLPs by cryoEM. VLPs were dissolved in borate-EDTA containing buffer, vitrified and analyzed by EM using a Tecnai F30 ‘Polara’ microscope (FEI) a Representative 2-D view of vitrified VLP, generated from repeated imaging each field of view along the Z- axis creating a series of images (constituting a movie). From ~300 movies, 6685 particles were selected for data processing. b Examples of generated 2-D classification, 20 were generated based on average electron density. White bar corresponds to 50 nm. Cross sectional Selection refinement towards 3-D electron density model and an initial model fitting data to icosahedral symmetry produced 9 Å model. c High level refinement further improved resolution to 4.2 Å. In addition, d the internal view reveals ordered elements (shown in orange) are visible on the interior of the capsid shell. e Resolution Fitting reconstruction reveals that the obtained structure corresponds to X-ray crystal structural data