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. 2017 Oct 23;7:13779. doi: 10.1038/s41598-017-13891-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional analysis of the DEKnull antigens. (a) Western blot analysis shows refolded recombinant DEKnull (D1), DEKnull-2 (D2) and Sal1 (S1) bind to DARC positive ( + ) but not DARC negative (−) red blood cells, while recombinant DEKnull-3 (D3) and DEKnull-4 (D4) do not bind to either erythrocyte type. DARC positive red blood cells (R) without bound antigen served as control (b) COS7 cell surface-expressed Sal1 and DEKnull-2 antigens bind to DARC positive red blood cells in the standard in vitro COS7 cell binding assay, while DEKnull-3 and 4 do not. The blot is cropped to improve clarity and conciseness of the figure. The Full blot is presented in Supplementary Figure S3.