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. 2017 Oct 23;7:13750. doi: 10.1038/s41598-017-13150-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SHG imaging of collagen reorganization in skin sample from an old WT mouse (same as in Fig. 1b and c). (a,b) SHG images of an old WT mouse skin at (a) 1.1 and (b) 1.35 stretch ratio, showing a network of collagen fibers. The positions of hair follicles are revealed as dark regions with no SHG signal. The traction is along the horizontal direction. Scale bar: 100 µm. (c,d) Orientation mapping of collagen fibers extracted from the SHG images. The color code is given in the insert. (e) Orientation histograms of the collagen fibers obtained from the SHG images at increasing stretch ratios (only 1 out of 2 histograms is displayed). (f) Variation of the entropy of the orientation histogram as a function of the stretch ratio. The red line corresponds to a linear fitting.