Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 23;7:13821. doi: 10.1038/s41598-017-14198-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization of cardiomyocytes differentiated from H9 hESCs. (a) Bright field images of H9 cells at day 0 (left panel) and differentiating cells at day 30 (right panel). Scale bar = 100 μm (b) Differentiated cardiomyocytes (hESC-CMs) at day 30 were subjected to immunofluorescent staining with antibodies against α-SA (red), cTnT (green), or MLC2a (yellow). Nuclei were stained with DAPI, and the merged images are shown. Scale bar = 20 μm. (c) Protein expression in undifferentiated H9 cells, hESC-CMs, and adult human left ventricular tissue (adult hLV) was assessed by Western blot analysis with antibodies against pluripotency markers (OCT4 and NANOG) and cardiomyocyte markers (α-SA, cTnT, MLC2a, MLC2v, SERCA2a and Cx43). (d) The mRNA levels of α-MHC and β-MHC in hESC-CMs and adult hLV were accessed by quantitative RT-PCR and the ratio of β-MHC/α-MHC mRNA levels was determined (n = 3). *P < 0.05. (e) Gene expression in hESC-CMs at day 30 and hESCs was accessed by quantitative RT-PCR with cardiomyocyte markers (GATA4, GATA6, and cTnT) and pluripotency markers (OCT4 and NANOG) n = 3. *P < 0.05. (f) Flow cytometry analysis of hESC-CMs at day 30 with antibodies against cardiomyocyte markers (cTnT, MLC2a, and α-SA) and pluripotency markers (TRA-1-60 and SSEA3) is shown.