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. 2017 Oct 23;7:13821. doi: 10.1038/s41598-017-14198-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electrophysiological recordings of Ca²⁺ channels in hESC-CMs. Superimposed current traces were recorded by applying 500-ms step depolarizing pulses in the voltage range of −50 to +50 mV from a holding potential of −50 mV under control condition (a) and in the presence of 10 µM Nifedipine (b). (c) I-V relationship of peak current in the absence (open circle) and presence of Nifedipine (closed circle). n = 6. (d) Expression of Cav1.1, Cav1.2, and Cav3.1 subtypes in H9 cells and hESC-CMs was accessed by RT-PCR. n = 5. *P < 0.05.