Table 5.

Medium-long-chain fatty acids profile and fat proportion in milk according to feeding sequence on day 21^1–3.

Item	Feeding sequence			SEM	P-value
	2 C	LH	HL
Fat and fatty acid value (%)
C10:0	0.365a	0.342ab	0.313b	0.009	0.081
C12:0	0.480	0.479	0.483	0.007	0.968
C14:0	4.183	4.126	4.234	0.061	0.780
C14:1	0.359a	0.293a	0.135b	0.020	<0.0001
C15:0	0.111b	0.113b	0.294a	0.018	<0.0001
C16:0	32.75	31.68	31.24	0.414	0.334
C16:1	12.12	11.56	11.34	0.271	0.495
C17:1	0.101	0.108	0.117	0.013	0.876
C18:0	1.475	0.925	1.548	0.018	0.338
C18:1cis	2.892b	3.512a	3.185ab	0.414	0.005
C18:1trans	21.96b	26.95a	27.44a	0.271	<0.0001
C18:2cis, n − 6	17.68	17.90	18.14	0.013	0.757
C18:3, n − 3	1.664a	1.343b	1.437b	0.032	<0.0001
C20:2	0.201b	0.285a	0.210b	0.017	0.023
C22:6, n − 3	0.232a	0.148b	0.161b	0.007	<0.0001
MUFA	38.45b	42.42a	42.21a	0.374	<0.0001
PUFA	20.27	19.67	19.95	0.223	0.590
SFA	40.09	37.76	38.19	0.505	0.137
n − 6	18.12	17.90	18.14	0.199	0.877
n − 3	1.941a	1.491b	1.598b	0.036	<0.0001
Fat	6.484b	7.633a	6.755b	0.167	0.012
The ratio of fatty acids
C18:1cis/C18:1trans	0.131	0.132	0.117	0.003	0.091
n − 6/n − 3	9.386c	12.07a	11.37b	0.178	<0.0001

^a–cValues with different letters within the same row are different (P < 0.05).

¹2C = fed control diet at 0600 h and 1500 h a day, respectively, LH = fed low protein diet at 0600 h and high protein diet at 1500 h a day, HL = fed high protein diet at 0600 h and low protein diet at 1500 h a day, respectively.

²The percentage values are proportions of each individual fatty acid relative to the total detectable lipid in the sample.

³n = 10.