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. 2017 Oct 23;7:13749. doi: 10.1038/s41598-017-13664-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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W-2b triggers Chk2 activation in cancer cells. (A) MCF7 and HCT-116 cells were treated with vehicle and increasing concentrations of W-2b for 48 h; whole cell lysates were prepared and subjected to western blot analysis for the expression of pChk2 (T68), Chk2 and β-actin. (B) Densitometry analysis of the bands obtained from above experiments. Data are representatives of three independent experiments. Error bars: mean ± s.d. *P < 0.05, **P < 0.01.