Table 1.

Estimated energy landscape parameters and reaction kinetics of homophilic Dsg2 interactions in equilibrium.

Dsg2	${\mathit{x}}^{\mathbf{‡}}$ [nm]	${\mathit{k}}_{\mathbf{-}}^{\mathbf{0}}$[s−1]	$\mathit{\tau }$ [s]	${\mathit{k}}_{\mathbf{+}}^{\mathbf{0}}$[M−1s−1]	${\mathit{K}}_{\mathbf{d}}$ [μM]	$\mathbf{\Delta }{\mathit{G}}_{\mathbf{0}}$ [kJ mol−1]	$\mathbf{\Delta }{\mathit{G}}_{\mathbf{-}}^{\mathbf{‡}}$ [kJ mol−1]	$\mathbf{\Delta }{\mathit{G}}_{\mathbf{+}}^{\mathbf{‡}}$ [kJ mol−1]
Wildtype	0.70(10)	2.99(43)	0.33(5)	7270(1041)	412	18.99(353)	69.05(35)	50.06(318)
p.D105E (p.D154E)	1.09(19)	0.30(17)	3.39(190)	361(202)	817	17.32(67)	74.70(136)	57.38(70)
p.V343I (p.V392I)	0.91(14)	0.58(20)	1.73(60)	573(198)	1008	16.81(136)	73.06(84)	56.25(51)

Reaction length $x^{‡}$, kinetic rate constants of dissociation $k_{-}^0$ and association $k_{+}^0$, bond lifetime $\tau$, free energy $\mathrm{\Delta }{G}_0$, equilibrium constant of dissociation $K_{\mathrm{d}}$, activation energy of dissociation $\mathrm{\Delta }{G}_{-}^{‡}$ and activation energy of association $\mathrm{\Delta }{G}_{+}^{‡}$.