Figure 2.

Removal of the pig brain. (a) Following resection of the scalp, the cranial bone was incised along the black dashed line taking care not to lacerate the meninges. (b) Once the cranial bone was removed, the meninges was incised along the yellow dashed line using a surgical scissors and taking care not to lacerate the brain tissue. The brain was then separated from the optic nerves, olfactory bulbs, and surrounding connective tissue. The brain is finally removed from the skull and placed in phosphate buffer saline prior to testing.