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. 2017 Oct 23;7:13743. doi: 10.1038/s41598-017-13915-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Recognition of BVDV-1 proteins by T cells from immune cattle. PBMC from BVDV-immune cattle challenged by experimental infection with BVDV Oregon C24v (n = 5) were isolated on day 21 post-challenge, and stimulated in vitro with synthetic peptides pooled to represent BVDV-1 proteins. PBMC cultured in media alone were included as a negative control. IFN-γ secreted into culture supernatants was quantified by ELISA after 48 hours. Mean data are presented and error bars show the SEM. Values for virus and peptide pool-stimulated conditions were compared to the unstimulated (media) control using a one-way ANOVA followed by a Dunnett’s multiple comparison test; ****p < 0.0001, and *p < 0.05.