Figure 5.

Detection of BVDV in the blood of vaccinated animals following challenge infection. Following BVDV-1 challenge infection, blood samples from inactivated BVDV vaccine (IAV) (squares), Vaccine-NP (triangles) or Control-NP (circles) vaccinated calves were assessed for BVDV E^rns antigen by ELISA (A) and BVDV RNA by quantitative RT-PCR (B). Mean data for each group (n = 6) are presented, error bars represent SEM and statistical analysis was performed using a two-way ANOVA followed by a Tukey’s multiple comparison test; statistically significant differences between Control-NP group and the IAV and Vaccine-NP groups are indicated as ‘a’ and ‘b’, respectively. Horizontal dashed lines show the assay cut-off for antigen positivity (COD >0.3) and detection limit (3.55 TCID₅₀) for ELISA and RT-PCR assays, respectively.