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. 2017 Oct 23;7:13793. doi: 10.1038/s41598-017-14002-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular structure of monomeric water-soluble PFO. Crystal structure of PFO in a ribbon representation showing the four amino acid substitutions contained in the pPFO parental derivative. The E167C and C459A substitutions allow for a Cys specific labeling of the PFO derivative. The Y181A and F318A substitutions produce a non-lytic PFO derivative. Domains (D) are color coded and numbers indicated (PDB ID: 1PFO).