Figure 2.

In situ hybridization of Trp in hairy flower wasp C. prismatica adult female brain. (A,B) Frontal section of the brain hemisphere hybridized with antisense (A) and sense (B) Trp probes. Trp signals scattered outside MBs are marked with arrowheads in (A). (C) Magnified view of the MB shown in (A). (D) Illustration indicating the Trp signal strength and the calyx is overlaid to (C). Kenyon cells were divided into three regions based on Trp signal intensity and the location of the somata: the Inner core (with strong Trp signal, blue), middle (with weak Trp signal, yellow), and inner peripheral (with moderate Trp signal, red) regions. The calyx is outlined with red line. (E) Distributions of the diameter of the somata of the each region are visualized with beanplot package⁶⁰. Weak, Moderate and Strong correspond to regions colored in yellow, red, and blue in (D). MBC, mushroom body calyx; OL, optic lobe; AL, antennal lobe; “n = ”, number of the cells measured; asterisk, p-value < 0.01 (Welch’s t-test for Strong-Moderate, Student’s t-test for Weak-Moderate). Bars indicate 500 μm in (A) and (B), 100 μm in (C) and (D).