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. 2017 Oct 23;7:13785. doi: 10.1038/s41598-017-14174-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In situ hybridization of Trp in sawfly Arge similis adult female brain. (A,B,C,D,) Frontal section of the brain hemisphere hybridized with antisense (A,C) and sense (B,D) Trp probes. (A,B) An individual younger than three days after emergence. (C,D) An individual older than ten days after emergence. (E) Magnified view of the MB of a serial section of (A,B). Schematic illustration of the measurements are overlaid. One soma is marked with black circle as an example. Diameter of the somata and the distances (black straight line) from the center of the calyx (black dot) were measured. Somata within the region enclosed with grey line are used for measurements. (F) A scattered plot graph of the measurements explained in (E). Each dot represents one soma. “n = ”, number of the cells measured; MBC, mushroom body calyx; OL, optic lobe; SOG, suboesophageal ganglion. Bars indicate 200 μm in (A,B,C,D), 50 μm in (E).