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. 2017 Oct 23;7:13816. doi: 10.1038/s41598-017-14124-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FTY720 affects mTORC1 signaling and LAT1 activity. (A) T-REx HeLa cells were incubated with rapamycin (200 ng/ml) or FTY720 (5 μM) for the indicated times. Cell extracts were immunoblotted with anti-p70^S6K/p85^S6K and anti-phospho-p70^S6K/p85^S6K (Thr389). (B) HeLa cells were incubated for 3 min in the presence of 0.1 mM [¹⁴C]-leucine alone or 0.1 mM [¹⁴C]-leucine with 5 μM FTY720 or 2 mM BCH. Data are means of four independent experiments and error bars correspond to standard deviations (unpaired t-test, ***P < 0.001).