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. 2017 Oct 23;7:13850. doi: 10.1038/s41598-017-14120-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Skeletal muscle gene expression of branched chain amino acids mitochondrial proteins in type 2 diabetic subjects (T2D) and their respective matched control groups (C). Real-time PCR was performed in skeletal muscle biopsies: (a) BCAT2, (b) BCKDHA, and (c) BCKDHB); Plasma metabolomics was performed in the same subjects and relative values are shown: (d) Leucine, (e) Valine, (f) Isoleucine and catabolites (g) 4-methyl-2-oxopentanoate, (h) 3-methyl-2-oxobutyrate and (i) 3-methyl-2-oxovalerate. Data are presented as mean ± standard deviation. Statistical analyses comparing type 2 diabetic subjects vs respective controls were performed by unpaired t-test *p < 0.05.