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. 2017 Oct 23;7:13741. doi: 10.1038/s41598-017-14096-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of [³H]GLPG0974 to hFFA2 and various mutants. Total, nonspecific and specific (total minus nonspecific) binding of the indicated concentrations of [³H]GLPG0974 to membranes of Flp-In TREx 293 cells induced to express wild type hFFA2 (a), Lys⁶⁵Arg hFFA2 (b), Lys⁶⁵Ala hFFA2 (c) or Arg⁶⁵Lys mFFA2 (d) is shown from representative experiments. The ability of C3 (e) and compound 1 (f) to compete with approximate K_d concentrations of [³H]GLPG0974 to bind to membranes expressing wild type (●) or Lys⁶⁵Ala () hFFA2 is illustrated.

Inline graphic — Binding of [³H]GLPG0974 to hFFA2 and various mutants. Total, nonspecific and specific (total minus nonspecific) binding of the indicated concentrations of [³H]GLPG0974 to membranes of Flp-In TREx 293 cells induced to express wild type hFFA2 (a), Lys⁶⁵Arg hFFA2 (b), Lys⁶⁵Ala hFFA2 (c) or Arg⁶⁵Lys mFFA2 (d) is shown from representative experiments. The ability of C3 (e) and compound 1 (f) to compete with approximate K_d concentrations of [³H]GLPG0974 to bind to membranes expressing wild type (●) or Lys⁶⁵Ala () hFFA2 is illustrated.