Figure 5.

Activation of hFFA2 by C3 and by compound 1 induces TGFα shedding via both G_q/11 and G_12/13 (a,d) Varying concentrations of C3 (a) or compound 1 (d) were able to promote shedding of TGFα via hFFA2 in each parental HEK293 cells and those genome edited to lack (Δ) expression of G_q+G₁₁, or G₁₂+G₁₃ but not in cells genome edited to lack all four of these G protein α subunits (ΔΔ). (b,e) Reintroduction of each of Gα_q, Gα₁₁, Gα₁₂ and Gα₁₃ into cells lacking all four of these G proteins allowed reconstitution of TGFα shedding via FFA2 by both C3 (b) and compound 1 (e). (c,f) Introduction of chimeric Gα_q−12 or Gα_q−13 into HEK293 cells genome edited to lack each of Gα_q, Gα₁₁, Gα₁₂ and Gα₁₃ resulted in effective shedding of TGFα in response to C3 (c) and compound 1 (f).