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. 2017 Oct 23;7:13741. doi: 10.1038/s41598-017-14096-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence alignment of FFA2 orthologs. Clustal Omega alignments of the primary amino acid sequence of available orthologs of FFA2 using human residues 60 to 119 as reference. Whether Lys or Arg is present as residue 65 (location 2.60) is shown in color. Glu⁶⁸ (location 2.63) is fully conserved and Phe⁸⁹ (location 3.28) is also entirely conserved apart from in kangaroo rat, western clawed frog and channel catfish.