Table 1.

Lys⁶⁵Xaa alterations in hFFA2 affect both agonist potency to generate inositol monophosphates and the ability of antagonists to inhibit this response.

Compound	WT	K65R	K65A	K65E
C3	4.06 ± 0.08	3.71 ± 0.05*	<2.5	<2.5
Compound 1	6.98 ± 0.06	6.70 ± 0.07*	<5.5	<5.5
GLPG0974	7.92 ± 0.19	<4.5	7.36 ± 0.17	<4.5
CATPB	7.72 ± 0.14	<4.5	6.36 ± 0.19	<4.5

Values represent pEC₅₀ for agonists C3 and compound 1 and pIC₅₀ values for antagonists GLPG0974 and CATPB against an EC₈₀ concentration of compound 1. Data are mean ± SEM with n ≥ 3. ^*p ≤ 0.05. One-way analysis of variance was followed by Dunnett’s test with WT as reference.