Figure 6.

Summary of the CST Phenotypes. In control mice, Sulf1/2 and Slit2 proteins, produced by the radial glial cells, are present on the pial surface. In Sulf1/2 DKO mice, increased 6-O-sulfated HS results in excessive accumulation of Slit2 protein (shown in dark blue) in the posterior hypothalamus, leading to dorsal displacement of the CST. Because DKO mice die within a day of birth, the CST caudal to the pyramidal decussation was not examined (dashed lines). In Slit2 KO mice, the CST axons invade the ventral forebrain owing to the lack of repulsion by Slit2.