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. 2017 Oct 23;7:13772. doi: 10.1038/s41598-017-14226-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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2-NBDG and TMRE are chemically compatible. Solutions containing single-component or combined 2-NBDG and TMRE solutions with an internal standard (Hs10) were analyzed by LC-MS for possible chemical cross-reactivity. (a) Extracted ion chromatograms (EIC) showing 2-NBDG as a mixture of alpha- and beta-anomers, TMRE as a mixture of methyl and ethyl esters, and Hs10 as a single peak. Chromatographic features from combined solutions (2-NBDG + TMRE) and single-component solutions (2-NBDG or TMRE alone) were maintained. (b) The areas under the curves (AUC) for 2-NBDG (AUC_2-NBDG), TMRE (AUC_TMRE), and Hs10 (AUC_Std) were computed from summation of EI peak integrations related to each compound. Results are shown as the ratio of AUC_TMRE or AUC_2-NBDG normalized to AUC_Std.