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. 2017 Oct 23;7:13772. doi: 10.1038/s41598-017-14226-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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2-NBDG uptake is unchanged by simultaneous injection with TMRE, while TMRE uptake is significantly inhibited by simultaneous injection with 2-NBDG. 2-NBDG and TMRE kinetic imaging was performed on non-tumor murine dorsal window chambers after they received a simultaneous injection (2-NBDG + TMRE) or an independent injection (2-NBDG or TMRE alone). (a) Representative 2-NBDG uptake time course images for simultaneous injection and independent 2-NBDG injection. (b) Representative TMRE uptake time course images for simultaneous injection and independent TMRE injection. (c) Mean uptake kinetics of 2-NBDG. R_D refers to the rate of delivery of 2-NBDG. 2-NBDG_max = the peak intensity of 2-NBDG. T_max = time (in seconds) at which 2-NBDG_max occurs. (d) Mean uptake kinetics of TMRE. NS = not significant. N = 5 mice/group. Comparison of mean kinetic curves across animal groups was performed with a two-way Analysis of Variance (ANOVA) test using the MATLAB (Mathworks, USA) statistics toolbox.