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. 2017 Oct 23;7:13772. doi: 10.1038/s41598-017-14226-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simultaneous injection with glucose does not affect TMRE uptake, while simultaneous injection with 2-DG decreases TMRE uptake by half. TMRE kinetic imaging was performed on murine dorsal window chambers after they received simultaneous injection of TMRE and glucose, 2-DG, or 2-NBDG. (a) Representative TMRE uptake time course images for each group. (b) Mean uptake kinetics of TMRE for each group. (c) Statistical comparison of the mean kinetic curves for the simultaneous injection groups vs. TMRE alone. NS = not significant. N = 4–5 mice/group. Comparison of mean kinetic curves across animal groups was performed with a two-way Analysis of Variance (ANOVA) test using the MATLAB (Mathworks, USA) statistics toolbox.