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. 2017 Oct 23;7:13772. doi: 10.1038/s41598-017-14226-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of the fluorescence spectral imaging system with millimeter-scale field of view and micron-level resolution. The 488 nm laser was used for 2-NBDG imaging while the 555 nm laser was used for TMRE imaging. BX: Beam expander; BS: Beam splitter; CCD: Charge-coupled device; DBS: Dichroic beam splitter; LCTF: Liquid crystal tunable filter; OBJ: Objective lens; P: Polarizer; RL: Relay lens; SF: Spatial filter; SLM: Spatial light modulator; TL: Tube lens.