Figure 9.
Estimated in vivo concentrations of 2-NBDG and TMRE in normal and tumor window chambers using a hyperspectral imaging system44,45. Fluorescence images were captured in non-tumor (N.T.) window chambers and in 67NR, 4T07, and 4T1 murine tumors after single injection with 2-NBDG (0.1 mL of 6 mM) or TMRE (0.1 mL of 25 µM). The estimated 2-NBDG and TMRE concentrations were then calculated by comparing in vivo fluorescence intensities to fluorescence intensities of tissue-mimicking phantoms imaged with the same instrument settings. Numbers in the tables correspond to fluorescence intensities which were then converted to estimated tissue-level concentrations.