Table 1.

Animal imaging protocols.

Injection protocol	Time point and dosage of tail vein injection
Normoxia in normal animals
TMRE only	t = 0 min: 100 µL of 75 µM TMRE
2-NBDG only	t = 0 min: 100 µL of 6 mM 2-NBDG
TMRE + 2-NBDG	t = 0 min: 100 µL of 75 µM TMRE + 6 mM 2-NBDG
TMRE + Glucose	t = 0 min: 100 µL of 75 µM TMRE + 6 mM glucose
TMRE + 2-DG	t = 0 min: 100 µL of 75 µM TMRE + 6 mM 2-DG
TMRE → 2-NBDG (Delay: 10–15 mins)	t = 0 min: 100 µL of 75 µM TMRE; t = 10–15 min: 100 µL of 6 mM 2-NBDG
Hypoxia in normal animals
TMRE only	t = 0 min: 100 µL of 75 µM TMRE
2-NBDG only	t = 0 min: 100 µL of 6 mM 2-NBDG
Optimal strategy	Optimal strategy determined from normoxic imaging groups
4T1 tumors
Optimal strategy	Optimal strategy determined from normoxic imaging groups