Skip to main content
. 2017 Sep 15;8(3):179–184.

Fig. 1.

Fig. 1

Bactericidal activity of bovine serum on P. multocida. Data were the average of two assays ± standard deviation. An index of < 1 would indicate a bactericidal effect. The bactericidal index was defined as CFU mL-1 after incubation for 1 hr divided by CFU mL-1 at time zero. All three groups C1, D1, and E1 showed roughly the same growth rate and there was no significant difference between these average growth indexes (p > 0.05). A1: Immune serum + normal calf serum, B1: Heated immune serum + heated normal calf serum, C1: Immune serum + PBS, D1: Heated immune serum + PBS, E1: Normal calf serum + PBS, F1: Heated normal calf serum + PBS, and G1: PBS. Each well of a 96-well microtitre plate contained 25 μL of immune serum from a calf with high level of IgG and IgM antibody (Immune.Ser), with or without 25 μL of fresh normal calf serum (NC.Ser) or 25 μL heated Immune.Ser with or without 25 μL heated NC.Ser or 25 μL fresh NC.Ser or 50 μL heated NC.Ser or 50 μL PBS. A 50 μL suspension of P. multocida B:2 was added to each well and then incubated for 1 hr at 37 ˚C. Sample of 10 µL from undiluted, and dilutions of 10-1 and 10-2 were taken before and after incubation and were plated out on serum bovine albumin (SBA) and then incubated overnight