Fig. 2.
Titration of a prototype HCV H77 strain using three NAT assays for quantifying HCV RNA. The HCV H strain was tested by TaqMan (open circles), Versant HCV RNA 3.0 b-DNA (diamonds) and the HCV Monitor 2.0 (solid circles). Based on linear regression and titration values, the calculated quantity for the HCV H strain was 107.4, 107.5, and 107.7 IU/ml respectively by b-DNA, TaqMan and Amplicor Monitor, which correlated with the infectious titer (106.5 CID50/ml) of this strain as described by Feinstone et al. [1981] and Purcell (unpublished work). The slopes for b-DNA, Monitor and TaqMan (−0.975, −0.968, and −0.974) respectively, were not significantly different (P = 0.25).
