Table 1. Phenotypic and chemotaxonomic data of Oblitimonas alkaliphila gen. nov., sp. nov. and the nearest phylogenetic neighbours.
Strains: 1, B4199T; 2 (n=7), C6819, C6918, D2441, D3318, E1086, E1148 and E5571; 3, P. alcaliphila DSM 17744T; 4, P. caeni DSM 24390T; 5, P. pseudoalcaligenes ATCC 17440T; 6, Serpens flexibilis ATCC 29606T; 7, T. denitrificans X2T. All strains positive: catalase, oxidase, growth at 10 °C, 25 °C, 35 °C, pH 7.5, pH 8.0, pH 9.0, and in nutrient broth with 2% NaCl; all strains negative: TSI slant acid, TSI butt acid, gelatin and aesculin hydrolysis, growth with 10% NaCl, utilization of d-mannitol, lactose, sucrose, maltose. +, Positive; −, negative; ND, no data.
| Test performed | 1 | 2* | 3 | 4 | 5 | 6 | 7† |
|---|---|---|---|---|---|---|---|
| Oxidation of: | |||||||
| d-Glucose | + | 7(100) | + | − | − | − | − |
| d-Xylose | + | 7(100) | + | − | − | − | ND |
| Urea, Christensen’s | − | 0(0) | + | − | − | − | − |
| Nitrate reduction | − | 0(0) | + | − | + | + | + |
| H2S (lead acetate paper) | − | 0(0) | − | − | + | − | ND |
| Growth temperature: | |||||||
| 4 °C | − | 0(0) | − | + | − | − | − |
| 42 °C | + | 6(85.7) | − | − | − | + | − |
| 45 °C | − | 0(0) | − | − | − | + | − |
| Growth conditions: | |||||||
| Anaerobe Jar | − | 0(0) | + | + | + | + | + |
| Nutrient broth, 0% NaCl | + | 7(100) | + | − | + | + | + |
| Nutrient broth, 4% NaCl | + | 7(100) | + | − | + | + | − |
| Nutrient broth, 6% NaCl | + | 7(100) | + | − | + | − | − |
| Nutrient broth, 8% NaCl | − | 2(28.6) | + | − | − | − | − |
| Growth on: | |||||||
| MacConkey agar | + | 7(100) | + | − | + | + | ND |
| Salmonella Shigella agar | + | 7(100) | + | − | + | − | ND |
| Cetrimide agar | − | 0(0) | + | − | + | − | ND |
| Citrate utilization | − | 0(0) | + | − | + | − | − |
| Motility | − | 0(0) | + | + | + | + | + |
| Growth at pH: | |||||||
| 6.0 | + | 7(100) | + | − | + | − | + |
| 10.0 | + | 7(100) | + | − | + | + | + |
| 11.0 | + | 6(85.7) | + | − | + | − | − |
*
n=7, Number positive (%).
†
Data from Tan et al. (2015).