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In vitro characterization of the ionic strength sensors. (a) Fluorescence emission spectra of the RD sensor titrated with potassium chloride. (b) Dependence of the mCitrine/mCerulean3 ratio of the EK, RD, RK, and E6G2 sensors on the potassium chloride concentration. (c) Dependence of the mCitrine/mCerulean3 ratio of the RD sensor on the ion identity. All experiments are in 10 mM NaPi, at pH 7.4. Error bars indicate the standard deviation of three biological replicates.
