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. Author manuscript; available in PMC: 2017 Oct 24.
Published in final edited form as: Dev Cell. 2017 Sep 14;43(1):60–70.e5. doi: 10.1016/j.devcel.2017.08.012

Figure 4. Cross species incompatibility in Rhi-Del interaction.

Figure 4

(A, B) Mass spectrometric analysis of Rhi binding proteins. Graphs showing ratios of GFP normalized iBAQ values for mel-Rhi vs. sim-Rhi (A), and mel-Rhi vs. Sha chimera (B), ranked by ratio values. Transgenes were expressed in melanogaster using the germline specific nanos-Gal4 driver.

(C) Localization of THO2 (piRNA cluster marker), H3K9me3 marked chromatin in the germline nuclei expressing Act5C-Gal4 driven Rhi:GFP. Color assignments for merged image shown on top. Scale bar: 2μm. Fluorescence intensities are measured along the line shown in merged image for Rhi:GFP (green), THO2 (red) and H3K9me3 (blue) as depicted in (D).

(E) Localization of Act5C-Gal4 driven Rhi-Del fusion proteins with respect to THO2 (piRNA cluster marker) in the female germline nuclei. Scale bar: 2μm.

See also Figure S4.