Table 1.
Summary of merged sequencing data in a comparison between dCATCH-Seq and CATCH-Seq
| Experiment | Total reads | Aligned reads | Mean PCR duplicates (%) | Aligned reads on target | On-target rate (%) | Uniquely aligned readsa | Uniquely aligned reads on targeta | On-target rate (%)a | Mean coverage on target |
|---|---|---|---|---|---|---|---|---|---|
| dCATCH-Seq CATCH-Seq |
853,358 841,154 |
414,965 802,931 |
47.4 2.8 |
382,625 291,808 |
92.21 36.34 |
406,331 762,445 |
379,084 289,558 |
93.29 37.98 |
145.98 110.18 |
aUniqueness and relevant statistics are calculated from reads with mapping quality (MAPQ) ≥ 20