Figure 4.

Effects of HW on serum TARC levels and expression of TARC and AQP-3 mRNA. a) Serum concentrations of TARC were compared at 0 and 4 weeks of HW administration by ELISA. *p<0.05 (HW mice, 0 versus 4 weeks), **p<0.05 (PW versus HW at 4 weeks), ***p<0.05 (SPF versus HW at 4 weeks). b) Effects of HW on the expression of TARC and AQP-3 mRNA in AD skin lesions were analyzed by real-time qPCR after 4 weeks of HW administration. The results were normalized to GAPDH mRNA levels used as control; *p<0.05 (PW versus HW). All data are shown as the mean±SEM (n=4 mice per group). HW - hydrogen water, SPF - specific-pathogen-free, PW - purified water, TARC - thymus and activation-regulated chemokine, APQ-3 - aquaporin