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. 2017 Oct 23;10:71. doi: 10.1186/s13048-017-0366-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Isolation of human preantral follicles. In the previous protocol, some follicles showed extruded oocytes (red arrow) (a); in the modified protocol, the vast majority of follicles were isolated from ovarian tissue samples (b). Distribution of isolated follicles in the modified protocol according to the three time intervals (c); some follicles remained entrapped in fragments of undigested tissue (black arrows) (d)