Fig. 4.

Effects of PAE on the fractional synthesis of non-acetylated (NAc) vs acetylated (Ac) replication-dependent histone peptides in frontal cortex and hypothalamus of rat brain. We measured the incorporation of ²H in NAc H2BK9 (iLLPGELAkHAVSEGTkAVTkYTSAk), H3K1 (kQLATkAAR), and H4K5K9K13 (gkGGkGLGkGGAkR) and the Ac H2BK9ac (iLLPGELAkHAVSEGTkAVTkYTSAK), H3K1ac (kQLATkAAR), and H4K5acK9acK13ac (gkGGkGLGkGGAkR) in the frontal cortex (a, c, e) and the hypothalamus (b, d, f) of 6-day-old rat pups following a 5-day alcohol feeding and administration of ²H₂O. Histone Fractional Synthesis Rate (FSR) was calculated and data are shown as mean ± SE, n = 4 per group, *p < 0.05, **p < 0.01, AF vs PF; #p < 0.05, ## p < 0.01, ### p < 0.001 Nac vs Ac