Fig. 8.

Effects of PBN on fractional synthesis of histones in frontal cortex of PF and AF rat pups. We measured the incorporation of ²H in the canonical histone peptides H3 (kQLATkAAR), H4 (gkGGkGLGkGGAkR) and histone variant peptides H3.3 (kSAPSTGGVkkPHR), H2Az (aTIAGGGVIPHIHkSLIGkkGQQkTV), and H2Ax (kGHYAER) in frontal cortex of 6-day-old rat pups following a 5-day alcohol feeding and administration of ²H₂O and 100 mg/Kg of the free radical spin trap PBN (α-phenyl-N-tert-butyl nitrone). Histone Fractional Synthesis Rate (FSR) was calculated as described in the “Methods” section, and data are shown as mean ± SE, n = 5–8 per group, *p < 0.05, **p < 0.01, AF vs PF, #p < 0.05, ##p < 0.01, AF-PBN vs PF, AF and PF-PBN