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. 2017 Oct 23;9:117. doi: 10.1186/s13148-017-0416-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Effects of PAE on BrdU incorporation and cell proliferation in frontal cortex and hypothalamus of rat brain. Representative immunofluorescence images, at 20X, visualized using the fluorescence microscope Nikon Eclipse 2000 (Nikon, Melville, NY) are shown. Higher magnification of BrdU signal is shown in insets. BrdU positive cells were counted in frontal cortex (a) and hypothalamus (b) of PF and AF animals using ImageJ (National Institutes of Health) and quantifications are shown in (c). Data are shown as mean ± SE, n = 4–6 animals per group done in duplicates, *p < 0.05, ***p < 0.001, AF vs PF