Table 1.
List of histone peptides identified by LC-MS/MS and used to calculate turnover of unmodified and modified histone proteins
| Histone | Peptide sequence |
|---|---|
| H2Aun | pr vTIAQGGVLPNIQAVLLPk pr k prTESHHk |
| H2AK120meK126ac | pr vTIAQGGVLPNIQAVLLPk pr k meTESHHk ac |
| H2Bun | pr iLLPGELAk prHAVSEGTk prAVTk prYTSAK pr |
| H2BK9ac | pr iLLPGELAk acHAVSEGTk prAVTk prYTSAK pr |
| H3un | k prQLATk prAAR |
| H3K1ac | k acQLATk prAAR |
| H3K9 | k prSTGGk prAPR |
| H3K9me2 | k me2STGGk prAPR |
| H3K9me3 | k me3STGGk prAPR |
| H3K9ac | k acSTGGkAPR |
| H4un | pr g k prGGk prGLGk prGGAk prR |
| H4K5acK9acK13ac | pr gk prGGk acGLGk acGGAk acR |
| H1 | prrAk prAAk pr k prSTDHPk prYSDMIVAAIQAEk prNR |
| H1.1 | prsGVSLAALk pr k prSLAAAGYDVEk prNNSR |
| H1.4 | prsGVSLAALk pr k prSLAAAGYDVEk prNNSR |
| H1.5 | k prATGPPVSELITk prAVSASk prER |
| H3.3 | k prSAPSTGGVk pr k prPHR |
| H2Ax | k prGHYAER |
| H2Az | praTIAGGGVIPHIHk prSLIGk pr k prGQQkTV |
All N-termini and all Lysine residues were propionylated by chemical derivatization. me methyl, ac acethyl, pr propionyl amide